The Entourage Effect and Magic Mushrooms

Art Credit: Michael Runyon

When it comes to magic mushrooms, the world-famous psychedelic fungi, it’s the psilocybin molecule that often takes center stage.

Psilocybin is the most abundant psychoactive component in magic mushrooms, or “shrooms.” Research into the power of magic mushrooms often only uses this substance alone, rather than whole mushrooms. But we’ve known for over fifty years that magic mushrooms contain a number of other psychoactive compounds, including psilocin and baeocystin.

New research has shown that there are even more powerful compounds in magic mushrooms than we thought there were – and it’s possible they’re playing a crucial role in the psychedelic effects of shrooms.

This is known as an “entourage effect.” It’s well-known to exist with cannabis, but it’s something we’re still learning about when it comes to magic mushrooms.

So what are the extra compounds in magic mushrooms, and how are they working in tandem with psilocybin to give us a unique psychedelic experience?

Magic Mushrooms, Baeocystin, and More

The most abundant molecules in magic mushrooms are psilocybin, psilocin, and baeocystin.

Psilocybin is the one that typically draws the most attention. Magic mushrooms contain between 0.2-1.8% psilocybin by dry weight,[1] depending on the species. Once ingested, psilocybin is quickly converted to its active metabolite, psilocin. Both psilocybin and psilocin bind to serotonin receptors, and these twin troublemakers are responsible for the majority of the psychedelic effects of magic mushrooms.

As well as psilocin being created from psilocybin when you eat magic mushrooms, it is also found in magic mushrooms themselves. It comprises around 0.02-0.6% of the dry weight of magic mushrooms,[1] depending on the species.

Baeocystin is the next most abundant molecule in magic mushrooms, but we don’t know much about it. Like psilocin, it’s another derivative of psilocybin, so it’s likely to have some kind of psychoactive effect. Researcher Jochen Gartz has described how a small dose of 4mg provided him with “a gentle hallucinogenic experience.”[2] Baeocystin is present at around 0.005-0.36% in dried magic mushrooms,[1] depending on the species.

Beta-Carbolines Discovered in Magic Mushrooms

As well as these main players in magic mushroom psychopharmacology, some newcomers have just arrived on the scene.

Beta-carbolines are a class of alkaloids that are found in many plant species. Most notably, the plant medicine brew ayahuasca contains the beta-carbolines harmine, harmaline, and tetrahydroharmine, which block the monoamine oxidase (MOA) enzyme. Ayahuasca brews also often contain the psychoactive molecule DMT, which is normally destroyed by the MAO enzyme… You can see now how the beta-carbolines come into play; they block MAO and help the DMT survive longer in the body, where it can have a psychoactive effect.

Now, research has shown that at least four species of magic mushrooms also contain beta-carbolines: Psilocybe mexicana, Psilocybe cubensis, Psilocybe cyanescens, and Psilocybe semilanceata. The beta-carbolines harmane, harmine, norharmane and perlolyrine were found in all four species.[3]

Check out our guide to identifying magic mushrooms to see if you can find these species in the wild!

Interestingly, psilocin is also broken down in the body by the MAO enzyme, just like DMT is. And now we know that beta-carbolines are present in magic mushrooms, they could potentially be blocking the MAO enzyme! This could theoretically prevent psilocin from being broken down and deactivated, naturally extending or enhancing the psilocybin/psilocin experience.

Art Credit: Kit Mezeres

Are Magic Mushrooms Chemically Similar to Ayahuasca?

As we’ve mentioned, ayahuasca is a psychedelic plant brew that also contains beta-carbolines. They play an important role in its psychoactivity by helping the psychedelic molecule DMT survive longer in the body. This discovery that magic mushrooms also contain beta-carbolines could mean that ayahuasca and magic mushrooms are more similar than we thought!

But are there enough beta-carbolines in magic mushrooms to have a significant effect on your subjective experience?

Well, the most abundant of the beta-carbolines found in the species of magic mushrooms was harmane, which was present at between 0.2-21 ug/g of dried mass.[3] In ayahuasca brews, beta-carbolines are active at levels as low as 0.5ug for an average person.[4] This suggests that a moderate magic mushroom dose (around two grams of dried shrooms) would be more than enough for these beta-carbolines to contribute to the trip.

This could definitely be considered an entourage effect of magic mushrooms.

Research into Magic Mushrooms vs Psilocybin

Since most clinical research into magic mushrooms has focussed solely on psilocybin, there’s not much information out there comparing the subjective effects of psilocybin compared to whole mushrooms.

However, two studies in mice stand out.

One study from 2009 shows that when mice were given either psilocybin or magic mushroom extract, the magic mushroom extract was more effective at reducing anxious behaviors in the mice.[5] Another study, in 2015, found that magic mushroom extract was around ten times more effective at changing behavioral responses in mice compared to pure psilocin.[6]

This seems to confirm what researchers recently discovered when they saw beta-carbolines in magic mushroom species; that the entourage effect in magic mushrooms may help to extend or enhance the psychoactive powers of psilocybin and its derivatives.

Art Credit: Autumn Skye

Is There Only One Kind of Magic Mushroom Experience?

Everything we currently know about magic mushrooms tells us that there is a big difference between psilocybin and magic mushrooms.

We also know that different species of magic mushrooms can vary dramatically in the relative quantities of active compounds they contain; just like different strains of cannabis, or different recipes of ayahuasca.

We even know that different parts of the same mushroom can have different quantities of compounds – in some species, the caps can contain twice as much psilocybin as the stems![7] We also know that magic truffles contain different quantities of psilocybin compared to magic mushrooms.

So what does this mean for our understanding of the magic mushroom experience?

Basically, we’ve been oversimplifying all this time.

Almost all the clinical human research into the healing potential of magic mushrooms has used isolated psilocybin or psilocin instead of whole mushroom extracts. Indeed, the current regulatory system means that it’s very hard to get approval to test organic substances that may contain a variety of unstudied compounds.

So whenever we read research on psilocybin or psilocin alone, it’s good to remember that magic mushrooms are more complex than just one molecule.

And, whenever we trip on magic mushrooms, keep in mind that the experience we’re about to have will be colored by the specific melodies and harmonies of pharmacological interactions lying in wait inside the individual mushrooms we’ve picked or grown.



[2] Gartz J (1991). Further Investigations on Psychoactive Mushrooms of the Genera Psilocybe, Gymnopilus and Conocybe. Ann. Mus. Civ. Rovereto 7, p265–74.

[3] Blei F, Dörner S, Fricke J, Baldeweg F, Trottmann F, Komor A, Meyer F, Hertweck C & Hoffmeister D (2019). Simultaneous Production of Psilocybin and a Cocktail of β-Carboline Monoamine Oxidase Inhibitors in ‘Magic’ Mushrooms. Chem Eur J 10.1002/chem.201904363

[4] Callaway JC, Raymon LP, Hearn WL, McKenna DJ, Grob CS, Brito GS & Mash DC (1996). Quantitation of N,N-dimethyltryptamine and harmala alkaloids in human plasma after oral dosing with ayahuasca. Journal of Analytical Toxicology 20(6), p492-497.

[5] Matsushima Y, Shirota O, Kikura-Hanajiri R, Goda Y & Eguchi F (2009). Effects of Psilocybe argentipes on Marble-Burying Behavior in Mice. Biosci Biotechnol Biochem 73(8), p1866-8.

[6] Zhuk O, Jasicka-Misiak I, Poliwoda A, Kazakova A, Godovan VV, Halama M & Wieczorek PP (2015). Research on acute toxicity and the behavioral effects of methanolic extract from psilocybin mushrooms and psilocin in mice. Toxins (Basel) 27;7(4), p1018-29.

[7] Bigwood J & Beug MW (1992). Variations in psilocybin and psilocin levels with repeated flushes (harvests) of mature sporocarps of Psilocybe cubensis (Earle) Singer. Journal of Ethnopharmacology 5, p287-291.

About Patrick Smith

Patrick Smith is a biologist and writer who has been working in the psychedelic community for several years. Twitter: @rjpatricksmith

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